Plasmid

Part:BBa_K4305001:Design

Designed by: Woo Rin Lee   Group: iGEM22_Korea_HS   (2022-09-19)


pET28a-TFAM


Assembly Compatibility:
  • 10
    INCOMPATIBLE WITH RFC[10]
    Illegal XbaI site found at 5030
  • 12
    COMPATIBLE WITH RFC[12]
  • 21
    INCOMPATIBLE WITH RFC[21]
    Illegal BglII site found at 4964
    Illegal BamHI site found at 5134
    Illegal XhoI site found at 5758
  • 23
    INCOMPATIBLE WITH RFC[23]
    Illegal XbaI site found at 5030
  • 25
    INCOMPATIBLE WITH RFC[25]
    Illegal XbaI site found at 5030
    Illegal NgoMIV site found at 137
    Illegal NgoMIV site found at 3184
    Illegal NgoMIV site found at 3344
    Illegal NgoMIV site found at 4932
  • 1000
    INCOMPATIBLE WITH RFC[1000]
    Illegal SapI.rc site found at 2263


Design Notes

TFAM (43aa - 246aa) protein is expressed.


Source

TFAM (Human gene) https://www.ncbi.nlm.nih.gov/gene?Db=gene&Cmd=DetailsSearch&Term=7019

References